matlab-based automated netquant application (MathWorks Inc)
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Matlab Based Automated Netquant Application, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab-based automated netquant application/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
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1) Product Images from "Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations"
Article Title: Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations
Journal: Heliyon
doi: 10.1016/j.heliyon.2023.e16982
Figure Legend Snippet: Comparison of NET quantification by hand counting semi-automated quantification and NETQUANT. A : Cell numbers for Image set 1 determined by all three methods, showing significantly fewer cell counts by NETQUANT in the majority of the images. B : NET formation determined by all methods revealed a significant difference between hand counting and NETQUANT as well as the semi-automated method. Statistical analysis was performed by Two-Way ANOVA with Dunnett correction for multiple comparison. Semi-automated and NETQUANT values were compared with hand counting. Data are given as mean ± SD. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Techniques Used: Comparison
Figure Legend Snippet: Comparison of hand counting, NETQUANT, and semi-automated quantification for image set 2. A shows the cell count for the control group with the semi-automated methods giving unrealistically high values for some images. Significant differences by both semi-automated and NETQUANT compared to hand counting were apparent. In B, the pattern remained similar to A, where the semi-automated method showed remarkably higher values of cell counts in several cases. C depicts NET formation values by all three methods, with the semi-automated method showing significantly higher results than both other methods in the control group, even exceeding 100% NET formation. D shows NET formation of the stimulated group where NETQUANT exhibited values within the range of hand counting, while the semi -automated method revealed significant differences. Statistical analysis was performed by Two-Way ANOVA with Dunnett correction for multiple comparison. Semi -automated and NETQUANT values were compared to hand counting. Data are given as mean ± SD. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Techniques Used: Comparison, Cell Counting, Control